the inflammatory microenvironment, and the expression of bone-related growth factors in each group by qPCR. Results The concentrations of TGFβ1 and IGF-1 in CGF+DMEM medium were signifcantly higher than those in DMEM medium and CGF precipitation solution (P<0.01). The hPDLCs cells were relatively uniform, with no obvious impurity cells, stable morphology and vigorous growth. After further LPS and CGF intervention on hPDLCs, compared with the Con group, ALP decreased signifcantly in the LPS group, while the concentration of ALP increased after CGF intervention, even higher than the Con group; the ALP activity of the CGF group was signifcantly higher than that of the control group and the LPS group, and that in the LPS group was lower than that in the control group and the in?ammation group (P<0.01). Under the in?ammatory microenvironment, the expression levels of osteogenic markers ALP, COL1 and OCN in hPDLCs of CGF group were signifcantly higher than those of Con group and LPS group (P<0.01). Conclusion CGF has a positive e?ect on improving the osteogenic inhibition of human periodontal ligament cells in the in?ammatory microenvironment. Meanwhile, it can increase the levels of osteogenic related factors (ALP, COL1, OCN) and promote the expression of transcription factors related to osteogenic di?erentiation, so as to e?ectively repair the osteogenic inhibition of human periodontal ligament cells in the in?ammatory microenvironment.