Objective To explore the intracellular signal and effect of echinacoside in the process of heterogeneous scar repair. Methods A total of 120 Sprague-Dawley rats were randomly divided into control group (n=60) and study group (n=60). Through wound-induced heterogeneous scar formation, the control group received placebo treatment, and the study group received echinacoside treatment. The wound healing rate was observed at a specific time point, and tissue samples were collected for histological analysis. Molecular biology techniques were used to analyze inflammatory markers, angiogenesis, matrix metalloproteinase activity, and apoptosis. Results The wound healing time of the study group was (8.48±1.13) d, which was shorter than (11.35±1.57) d of the control group, and the difference was statistically significant (P<0.05). The degree of inflammation, vascular density and collagen deposition area in the study group were better than those in the control group, and the differences were statistically significant (P<0.05). The levels of leukocyte infiltration, TNF-α and IL-6 in the study group were lower than those in the control group, and the differences were statistically significant (P<0.05). The number of CD31 positive blood vessels and the expression level of VEGF in the study group were higher than those in the control group (P<0.05). Conclusion Echinacoside has a definite effect in promoting the repair process of heterogeneous scars, and the repair effect is good. Meanwhile, it can effectively promote wound healing, reduce the inflammatory response of scar tissue and accelerate vascular growth.