Objective To study the effect of xenogeneic collagen matrix and autologous connective tissue graft flap on periodontal soft tissue increment. Methods From July 2022 to December 2023, 80 patients with periodontal disease who underwent implant restoration or postoperative review in the medical electronic database of our hospital were selected as the research objects. According to the random number table method, patients were divided into the control group (n =40) and an observation group (n =40). The control group was treated with autologous connective tissue transplantation flap, and the observation group was treated with xenogeneic collagen matrix combined with autologous connective tissue transplantation flap. The wound healing time, clinical efficacy, aesthetic satisfaction, periodontal health indicators [modified sulcus bleeding index (mBI) and modified plaque index (mPI)] and complication were compared between the two groups. Results The wound healing time of the observation group was shorter than that of the control group [(14.01±2.10) d vs. (16.28±3.21) d] (P <0.05). The total effective rate of treatment in the observation group was higher than in the control group(97.50% vs. 82.50%) (P <0.05). The aesthetic satisfaction in the observation group was higher than that in the control group (P <0.05). The observation group had smaller mBI, mPI and incidence of complications in the observation group than those in the control group (P <0.05). Conclusion The combined treatment of xenogeneic collagen matrix and autologous connective tissue graft flap can improve the clinical effect, reduce the incidence of complications, shorten the wound healing time, improve the periodontal health index, and improve the aesthetic satisfaction of patients.