Objective To explore the effect of moist exposed burn ointment combined with epidermal growth factor after carbon dioxide fractional laser surgery. Methods A total of 102 patients who received carbon dioxide lattice laser treatment in the Department of Dermatology, the First People's Hospital of Hechi from November 2021 to December 2022 were randomly divided into control group and observation group, with 51 patients in each group. The control group was given routine intervention after operation, and the observation group was given moist exposed burn ointment combined with epidermal growth factor gel for intervention after operation. The pain degree, skin barrier function, scar condition and quality of life were compared between the two groups. Results After treatment, the NRS score of the observation group was lower than that of the control group (P <0.05). After treatment, the pH value, cuticle water content, a value and TEWL of the observation group were better than those of the control group (P <0.05). The color, thickness, softness and vascular distribution of scar in the observation group were better than those in the control group (P <0.05). The scores of all dimensions of quality of life in the observation group were higher than those in the control group (P <0.05). Conclusion The application of moist exposed burn ointment combined with epidermal growth factor gel after carbon dioxide fractional laser surgery is effective. After treatment, the pain of patients is effectively reduced, and the wound characteristics are improved, which is conducive to promoting the rapid healing of postoperative wounds and has positive significance for improving the quality of life of patients.