Objective To analyze the effect of triamcinolone acetonide assisted laser treatment of hyperplastic scar. Methods A total of 128 patients with hyperplastic scar admitted to Beijing Armed Police Corps Hospital from June 2021 to June 2024 were selected and divided into control group and observation group according to the different treatment schemes, with 64 patients in each group. The control group was treated with laser treatment alone, and the observation group was assisted by triamcinolone acetonide on the basis of the control group. The clinical efficacy, scar score, scar thickness, blood biochemical indexes [transforming growth factor β1 (TGF-β1), vascular endothelial growth factor (VEGF)] and adverse reactions were compared between the two groups. Results The total effective rate in the observation group was 95.31%, which was higher than 84.38% in the control group (P<0.05). The incidence of adverse reactions in the observation group was lower than that in the control group (P<0.05). After treatment, the scar score and scar thickness in the observation group were lower than those in the control group (P<0.05). After treatment, the TGF-β1 and VEGF in the observation group were lower than those in the control group (P<0.05). Conclusion The application effect of triamcinolone acetonide assisted laser treatment of hyperplastic scar is good, which can reduce patients' serum TGF-β1 and VEGF levels, inhibit hypertrophic scar formation, and can reduce the incidence of adverse reactions.