As an important product of genetic engineering, recombinant fibronectin has extensive application potential in the biological medicine. It shows good effects on skin anti-aging, scar repair and wound treatment by promoting collagen synthesis, enhancing cell adhesion, regulating inflammatory response and angiogenesis. Recombinant fibronectin can activate PI3K/Akt and Ras/MAPK signaling pathways, promote cell proliferation and differentiation, inhibit apoptosis, and provide support for tissue repair. In the field of medical aesthetics, recombinant fibronectin not only contributes to skin barrier repair and anti-aging, but also improves scar and promotes wound healing. The article was aimed at analyzing the study progress on anti-aging repair mechanism of recombinant fibronectin and its application in medical aesthetics, so as to optimize rFN expression system, explore its combination application and broaden its application field, and provide support for clinical treatment.