Objective To compare the elastic limits of two working lengths of polyether ether ketone with cobalt-chromium alloy, pure titanium and Vitallium 2000 clasp materials, and to analyze the loading force of each material when the deformation is 0.25 mm and 0.50 mm. Methods Four kinds of material clasp were prepared, 20 in each group. 10 of each group were randomly selected as clasp tip from the fulcrum 5 mm, and the remaining 10 as the clasp tip from the fulcrum 10 mm, so as to simulate the working lengths of the clasps in the premolar region and the molar region, respectively. The load-displacement test was performed with universal material tester, and the elastic limits and loading forces were compared among the four materials. Results There were significant differences in the elastic limits of the four materials between the clasp tip from the fulcrum 10 mm and the clasp tip from the fulcrum 5 mm (P <0.05). The elastic limit values decreased sequentially from polyether ether ketone, pure titanium, Vitallium 2000, and cobalt-chromium alloy. The elastic limit of the four materials in the clasp tip from the fulcrum 5 mm was lower than that in the clasp tip from the fulcrum 10 mm, and the difference was statistically significant (P <0.05). When the deformation was 0.25 mm, there was significant difference in the loading force of the four materials between the clasp tip from the fulcrum 10 mm and the clasp tip from the fulcrum 5 mm (P <0.05), and the loading force of the clasp in the polyether ether ketone group was smaller than that in the cobalt-chromium alloy group, pure titanium group and Vitallium 2000 group. When the deformation was 0.5 mm, there was significant difference in the loading force of the four materials between the clasp tip from the fulcrum 10 mm and the clasp tip from the fulcrum 5 mm (P <0.05), and the loading force of the clasp in the polyether ether ketone group was lower than that in the cobalt-chromium alloy group, pure titanium group and Vitallium 2000 group. Conclusion Compared with three commonly used removable partial denture metal clasp materials, polyether ether ketone has better elasticity, but it can provide less retention force under the same conditions, which can further increase the depth of undercut into the abutment, in order to obtain good retention force.