Objective: To analyze the clinical value of applying carbon dioxide fractional laser for cosmetic purposes in skin diseases. Method: A double-blind method was used to group 100 patients with skin diseases who were included in the study from December 2023 to December 2024 for research, with 1 group of 50 cases. The reference group was treated with traditional topical drugs, and the experimental group received carbon dioxide fractional laser cosmetic treatment. The degree of pain, the state of granulation tissue on the wound surface, the count of skin lesions, the indicators of skin barrier function and the effective rate of clinical treatment were compared between the two groups. Result: The indicators such as the effective rate of clinical treatment, nodules, papules, cysts, pustules, VAS score, color, texture, water content of the stratum corneum, skin PH value, and skin a value of the patients with skin diseases in the experimental group were more advantageous compared with those in the reference group, with P < 0.05, which was significant. Conclusion: The application of carbon dioxide fractional laser cosmetic therapy in skin diseases can help reduce the degree of pain and the count of skin lesions, improve the granulation state of the wound surface and the function of the skin barrier, and enhance the clinical efficacy.