Objective To explore the effect of platelet-rich plasma (PRP) therapy on wound healing, inflammatory factors and pain in patients with chronic wound healing. Methods A total of 120 patients with chronic wound admitted to the Department of Burns and Plastic Surgery, General Hospital of Southern Theater Command of the Chinese People’s Liberation Army from January to December 2023 were selected as the research subjects. According to the random number table method, they were divided into the control group and the observation group, with 60 patients in each group. The control group was treated with silver ion dressing, and the observation group was treated with PRP. The wound healing, inflammatory factor levels, pain indexes and satisfaction were compared between the two groups. Results The average number of dressing changes and the wound healing time in the observation group were lower than those in the control group, and the bacterial clearance rate and the granulation tissue coverage rate were higher than those in the control group (P <0.05). After treatment, the levels of IL-6 and IL-8 in the observation group were lower than those in the control group (P <0.05). After treatment, the scores of VAS, NRS and PPI in the observation group were lower than those in the control group (P <0.05). The satisfaction in the observation group was higher than that in the control group (P <0.05). Conclusion PRP therapy can promote the early wound healing of patients with chronic wound, inhibit the levels of inflammatory factors, relieve the pain during the wound healing period, and lead to a higher patient satisfaction.