Objective To analyze the effect of free latissimus dorsi bilobed flap repair technique on wound healing in patients with limb soft tissue defect. Methods A total of 40 patients with limb soft tissue defect admitted to the First Affiliated Hospital of Bengbu Medical University from July 2021 to July 2023 were selected as the research subjects. According to different treatment methods, they were divided into the control group and the study group, with 20 patients in each group. The control group received conventional soft tissue repair technique, and the study group received free latissimus dorsi bilobed flap repair technique. The postoperative flap repair, aesthetics and quality of life were compared between the two groups. Results The total flap survival rate in the study group was 90.00%, which was higher than 60.00% in the control group (P<0.05). The distal partial necrosis rate in the study group was 10.00%, which was lower than 40.00% in the control group (P<0.05). The direct suture rate of the donor site in the study group was 95.00%, which was higher than 65.00% in the control group (P <0.05). The free skin grafting rate in the study group was 5.00%, which was lower than 35.00% in the control group (P<0.05). The scores of blood vessel distribution, softness, scar thickness, scar color and VSS total score in the study group were better than those in the control group (P<0.05). The scores of physical, psychological, social and environmental dimensions in the study group were better than those in the control group (P <0.05). Conclusion The application of free latissimus dorsi bilobed flap repair technique in patients with limb soft tissue defect can improve flap survival rate, meet patients' aesthetic needs, enhance the wound repair quality, and improve the quality of life.