Objective To investigate the efficacy of fractional laser-assisted transdermal drug delivery in the treatment of neurodermatitis and its influence on patient satisfaction. Methods A total of 86 patients with neurodermatitis admitted to Xiangcheng District Yuanhe Street Community Health Service Center from April 2022 to October 2023 were selected as the research subjects, and they were divided into group A (43 patients) and group B (43 patients) according to the rando m number table method. Group A was given conventional transdermal drug delivery treatment, and group B was given fractional laser treatment before group A' s treatment. The clinical efficacy, skin lesion score and satisfaction were compared between the two groups. Results The total effective rate of treatment in group B was 97.67%, which was higher than 86.05% in group A (P <0.05). The skin lesion score of the two groups after 14 days of treatment was lower than that before treatment, and that of group B was lower than that of group A (P <0.05). The satisfaction rate of group B was 100.00%, which was higher than 90.70% of group A (P <0.05). Conclusion The fractional laser-assisted transdermal drug delivery in the treatment of neurodermatitis has a definite treatment effect, which can effectively improve skin lesion symptoms and improve patients' aesthetic satisfaction. It is worthy of clinical application.