Objective: To analyze the clinical effect of CO2 dot matrix laser combined with moist burn ointment in the treatment of hypertrophic scars. Method: 100 patients with hypertrophic scars admitted to the First Hospital of Longyan City, Fujian Province (from January 2022 to December 2024) were randomly selected as the research sample and divided into two groups according to the envelope method. The control group and the observation group were each divided into 50 cases. The control group was treated with CO2 fractional laser, while the observation group was treated with CO2 fractional laser combined with moist burn ointment. The wound pain score, scar hyperplasia score, incidence of adverse reactions, and time to complete epithelialization of the wound were compared between the two groups before and after 4.5 months of treatment. Result: Before treatment, there was no statistically significant difference in the wound pain score and scar hyperplasia score between the two groups of patients (P>0.05). After 4.5 months of treatment, the wound pain score (2.06 ± 0.53) and scar hyperplasia score (1.02 ± 0.08) in the observation group were lower than those in the control group (5.18 ± 0.97) and (3.84 ± 0.21), respectively. The incidence of adverse reactions in the observation group was 2.00% lower than that in the control group (10.00%), and the time for complete epithelialization of the wound in the observation group (5.13 ± 0.62) days was shorter than that in the control group (10.25 ± 1.08) days. The difference between the two groups was statistically significant (P<0.05). Conclusion: The clinical effect of CO2 dot matrix laser combined with moist burn ointment in the treatment of hypertrophic scars is significant. It not only reduces the pain and degree of scar hyperplasia in patients, but also accelerates wound recovery with few adverse reactions. It is an efficient and safe combination therapy.