Objective To study the repairing effect of a sterile skin repair patch added with sodium hyaluronate and ectoin on skin damage after medical aesthetic treatment through different evaluation methods. Methods An in vitro skin model was used to measure the inhibitory effect of the sterile skin repair patch on inflammatory factors and inflammatory mediators, as well as the promoting effect on the secretion of filaggrin (FLG) and loricrin (LOR). Subjects were given the patch after hydrating needle and radiofrequency treatments, and VISIA red zone images were taken to compare and verify the soothing and repairing effect of the sterile skin repair patch on skin damage after medical aesthetic treatment. Results The levels of IL-1α and TNF-α in the negative control group were significantly higher than those in the blank control group (P <0.01). The levels of IL-1α and TNF-α in the sample group were significantly lower than those in the negative control group (P <0.01). The inhibition rates of IL-1α and TNF-α in the sample group were 9.68% and 28.25%, respectively. The contents of LOR and FLG in the negative control group were significantly lower than those in the blank control group (P <0.01). The contents of LOR and FLG in the sample group were higher than those in the negative control group (P <0.01). The promotion rates of LOR and FLG in the sample group were 310.53% and 46.15%, respectively. After hydrating needle treatment, 9 out of 14 subjects who used the sterile skin repair patch showed lighter red zone color and smaller area compared with those before treatment. After radiofrequency treatment, 8 out of 11 subjects showed lighter red zone color and smaller area. No adverse reactions occurred in the subjects, the soothing and repairing effect was definite, and the overall satisfaction with moisturizing and repair reached 100.00%. Conclusion The sterile skin repair patch added with sodium hyaluronate and ectoin has a significant soothing and repairing effect on skin damage after medical aesthetic treatment, which can alleviate skin damage.