Objective To analyze the effect of microneedle introduction of pro-Xylane-containing liquid dressing on GAIS and VISIA scores in patients with facial rejuvenation. Methods A total of 60 patients with facial rejuvenation admitted to Yantai Yuhuangding Hospital from October 2024 to January 2025 were selected as the research subjects, and they were divided into the control group and the observation group by the random number table method, with 30 patients in each group. The control group was treated with pro-Xylane-containing liquid dressing alone, and the observation group was treated with microneedle introduction of pro-Xylane-containing liquid dressing. The GAIS score, VISIA score, pain degree and adverse reactions were compared between the two groups. Results The GAIS scores of the observation group at 2 and 4 weeks after treatment were (1.78±0.69)scores and (1.13±0.09)scores, respectively, which were lower than those of the control group [(2.36±0.78)scores and (1.75±0.69)scores] (P<0.05). The VISIA scores of spots, wrinkles, pores, texture and red areas in the observation group at 2 and 4 weeks after treatment were lower than those in the control group (P<0.05). The pain scores during and after operation in the observation group were (4.12±1.23)scores and (2.41±0.89)scores, respectively, which were higher than those in the control group [(1.12±0.75)scores and (0.78±0.36)scores] (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05).Conclusion The intervention scheme of microneedle introduction of pro-Xylane-containing liquid dressing can optimize the effect of facial rejuvenation, effectively improve the fineness of skin texture, reduce wrinkles, shrink pores, and has good safety during treatment.