Objective To explore the clinical effect of platelet-rich plasma (PRP) gel in the treatment of difficult-to-heal wound. Methods A total of 40 patients with difficult-to-heal wound admitted to Xingyi People's Hospital and Wound Stoma Clinic of Yunyan District Zhonghua Community Health Service Center from May 2020 to March 2025 were selected as the research subjects, and they were divided into the control group (20 patients) and the study group (20 patients) by the random number table method. The control group was treated with conventional debridement and dressing change, and the study group was treated with PRP gel. The wound healing time, wound volume reduction rate and bacterial culture positive rate were compared between the two groups. Results The average healing time of the study group was (26.58±4.94)days, which was shorter than that of the control group [(34.25±4.52)days] (P <0.05). The wound volume reduction rates of the study group at 7, 14 and 21 days after treatment were higher than those of the control group (P <0.05). The bacterial positive rate of the study group after treatment was lower than that of the control group (P <0.05). Conclusion PRP gel has a good effect in the repair of difficult-to-heal wound, which can accelerate wound repair, reduce wound volume, and lower the risk of infection.