Objective To explore the effect of mussel adhesive protein in the repair and treatment of sensitive skin. Methods A total of 220 patients with sensitive skin admitted to Xiamen Siming RAO Jialing Medical Beauty Clinic from January to December 2024 were selected, and they were divided into the control group and the study group by the random number table method, with 110 patients in each group. The control group received conventional treatment, and the study group received mussel adhesive protein treatment. The serum inflammatory factors, subjective symptom improvement, skin barrier function indicators, treatment effect and adverse reaction rate were compared between the two groups. Results The levels of TSLP and IL-3 in the study group were lower than those in the control group (P <0.05). The scores of heat sensation, pricking sensation, itching sensation and tightness in the study group after treatment were lower than those in the control group (P <0.05). The skin moisture content in the study group after treatment was higher than that in the control group, and the sebum content and TEWL were lower than those in the control group (P <0.05). The total effective rate of the study group (95.45%) was higher than that of the control group (65.45%) (P <0.05). The incidence of adverse reactions in the study group (4.55%) was lower than that in the control group (7.27%), but the difference was not statistically significant (P >0.05). Conclusion The application of mussel adhesive protein in the treatment of sensitive skin repair is effective, which can effectively improve patients' subjective symptoms, reduce serum inflammatory factor levels, help to promote the recovery of skin barrier function, and does not increase the risk of adverse reactions after treatment, with good application safety.