Objective To explore the effect of platelet-rich plasma combined with microneedle introduction of minoxidil in the treatment of patients with androgenetic alopecia. Methods A total of 70 patients with androgenetic alopecia admitted to the People's Hospital of Meitan and the Affiliated Hospital of Guizhou Medical University from October 2024 to March 2025 were selected, and they were divided into the control group (35 patients) and the study group (35 patients) by the random number table method. The control group was treated with microneedle introduction of minoxidil, and the study group was treated with platelet-rich plasma combined with microneedle introduction of minoxidil. The treatment effect, trichoscopic indicators and sex hormone levels were compared between the two groups. Results The total effective rate of treatment in the study group (97.14%) was higher than that in the control group (77.14%) (P <0.05). After treatment, the hair follicle density and hair density in the study group were higher than those in the control group, while the single-hair follicle proportion and vellus hair proportion were lower than those in the control group (P <0.05). After treatment, the estradiol level in the study group was higher than that in the control group, and the testosterone level was lower than that in the control group (P <0.05). Conclusion The combination of platelet-rich plasma on the basis of microneedle introduction of minoxidil has a more definite effect in the treatment of androgenetic alopecia, which can effectively regulate patients' sex hormone levels and improve trichoscopic indicators.