Objective To explore the efficacy of extracellular matrix repair in anti-wrinkle and firming of human skin. Methods From September to October 2024, 30 healthy women aged 30-40 years in Beijing were selected as the research subjects. Palmitoyl tripeptide-5, hydrolyzed ginsenosides and oligopeptides were screened as core active ingredients, and a lotion product containing the above ingredients was formulated. The anti-wrinkle and firming efficacy was systematically and comprehensively evaluated from three dimensions: cell experiment, zebrafish model experiment and human efficacy experiment. Results Safety tests showed that the irritation score (IS) of the tested product in the chick embryo chorioallantoic membrane test was 0 (non-irritating), and there were no adverse reactions in the patch test of 30 subjects. In the cell experiment, compared with the control group, the contents of glycosaminoglycan (GAG), elastin (ELN), type Ⅰ collagen, type Ⅲ collagen and transforming growth factor (TGF-β) in HFF-1 cells of the experimental group with the product added were increased by 32.80%, 60.64%, 16.47%, 20.79% and 13.11%, respectively (P <0.05). In the zebrafish experiment, the tested product could increase the relative expression levels of zebrafish elastin-related gene (eln1) and type Ⅰ collagen-related gene (col1a1a) by 108.77% and 20.61%, respectively (P <0.05). Human self-assessment results showed that after 4 weeks of use by 30 subjects, six evaluation indicators including skin hydration, firmness and wrinkle reduction were improved. Conclusion Palmitoyl tripeptide-5, hydrolyzed ginsenosides and oligopeptides have definite anti-wrinkle and firming effects and good safety, and can be used as effective active ingredients in the development of anti-wrinkle cosmetics.