Objective To analyze the effect of fractional micropore laser combined with epidermal growth factor on skin barrier function in patients with facial acne depressed scar. Methods A total of 88 patients with facial acne depressed scar admitted to Jiangbei District Waitan Street Community Health Service Center from March 2023 to March 2025 were selected, and they were divided into the control group and the study group by the random number table method, with 44 patients in each group. The control group was treated with single fractional micropore laser, and the study group was treated with fractional micropore laser combined with epidermal growth factor. The skin barrier function, ECCA scores and recovery time of clinical symptoms were compared between the two groups. Results At 3 and 6 weeks after treatment, the skin sebum content, skin moisture content and transepidermal water loss in the study group were better than those in the control group (P <0.05). The ECCA scores in the study group at 3 and 6 weeks after treatment were lower than those in the control group (P <0.05). The incrustation time, edema time and scar descrustation time in the study group were shorter than those in the control group (P <0.05). Conclusion Fractional micropore laser combined with epidermal growth factor in the treatment of patients with facial acne depressed scar can effectively improve skin barrier function, reduce the severity of acne scars, and help to shorten the recovery time of clinical symptoms.