Objective To analyze the effect of medical membrane wound functional dressing combined with microneedling on follicle repair and hair growth in patients with hair follicle damage. Methods A total of 75 patients with hair follicle damage admitted to Shaoshan People's Hospital from October 2023 to April 2024 were selected, and they were divided into group A, group B and group C by the random number table method, with 25 patients in each group. Group A was treated with conventional gauze, group B was treated with medical membrane wound functional dressing, and group C was treated with microneedling combined with medical membrane wound functional dressing. The follicle repair status, hair growth status, wound healing indicators and inflammatory marker levels were compared among the three groups. Results The follicle regeneration rate and follicle repair speed of group C were better than those of group A and group B (P <0.05). The hair growth density, hair diameter change and hair growth speed of group C were better than those of group A and group B (P <0.05). The wound healing time of group C was shorter than that of group A and group B, and the healing score were higher than those of group A and group B (P <0.05). The levels of IL-6, TNF-α and CRP in group C were lower than those in group A and group B (P <0.05). Conclusion The application of medical membrane wound functional dressing combined with microneedling in patients with hair follicle damage can promote follicle repair and regeneration, optimize hair growth, help to reduce the levels of inflammatory markers, and promote wound healing, which is worthy of clinical application.