Abstract: Objective To analyze the Demodex folliculorum (Df) infection status and risk factors in different subtypes of rosacea. Methods A total of 130 patients with rosacea diagnosed in the Dermatology Department of our hospital from June 2023 to June 2025 were enrolled in the study, including 57 patients with erythematoteleangiectatic rosacea (ETR), 53 patients with papulopustular rosacea (PPR), and 20 patients with hypertrophic rosacea (PHR). The Df infection status of patients with different subtypes of rosacea was statistically analyzed. Univariate and multivariate logistic regression analyses were conducted to summarize the independent risk factors for Df infection in rosacea patients. Results The Df positivity rate among the 130 rosacea patients was 64.62% (84/130), with a higher rate in PPR patients compared to other subtypes (P<0.05). There were statistically significant differences in age, disease duration, facial skin type, and sleep quality between Df-positive and Df-negative patients (P<0.05). Logistic regression analysis revealed that PPR subtype, age >31 years, disease duration >3 years, and oily skin were independent risk factors for Df infection in rosacea patients (P<0.05). Conclusion There are significant differences in Df infection status among patients with different subtypes of rosacea, and subtype type, age, disease duration, and skin type are all influencing factors for Df infection in patients.